|
Document Repository (Public)
|
These reports are the confidential property of the Pine Chemicals Association and/or one or more of its members.
Unauthorized reproduction or distribution of it is PROHIBITED.
Please Note:
Some files are large and may take several seconds to download.
Use your browswer's Back button to navigate to the previous page without closing the window.
|
Document Title: Rosin, Partially Hydrogenated methyl ester, CAS No.8050-15-5,Testing for Mutagenic Activity with Salmonella typhimurium TA 1535, TA 1537, TA 98 and TA 100 and Escherichia coli WP2uvrA
|
Abstract:
Rosin, Partially Hydrogenated methyl ester, CAS No. 8050-15-5, was tested for mutagenic activity in Salmonella typhimurium strains TA 1535, TA 1537, TA 98 and TA 100 and Escherichia coli WP2uvrA at concentrations ranging from 17 to 5000 ?g per plate. The solvent used was ethanol. Two mutation assays and a repeat test with TA 1537 and WP2uvrA were conducted on agar plates in the presence and absence of an Aroclor 1254 induced rat liver preparation and the co-factors required for mixed-function oxidase activity (S9 mix). Concurrent positive controls demonstrated the sensitivity of the assay and the metabolising activity of the S9 mix. No mutagenic activity was observed in any of the 5 bacterial strains, in either activation condition. Precipitation of the test item was observed in both mutation assays at 1667 and 5000 ?g per plate, in both activation conditions. There was no toxicity to the bacteria, although at 5000 ?g per plate, the precipitate was too dense to assess accurately the condition of the background lawns of microcolonies. However, the lawns were assumed to be normal. It was concluded that Rosin, Partially Hydrogenated methyl ester, CAS No. 8050-15-5, was not mutagenic to Salmonella typhimurium or Escherichia coli when tested in ethanol up to a predetermined maximum limit of 5000 ?g per plate.
|
Download Document: Viewing this document is limited to PCA Membership Only
|
|
Author: Stevenson F M - Provided by: Walter Jones - ID: 100.IR.20337 - Date: 12/13/2001
Providing Organization: Pine Chemicals Association |
Document Title: Tall Oil, CAS No. 8002-26-4 Testing for Mutagenic Activity with Salmonella typhimurium TA 1535, TA 1537, TA 98 and TA 100 and Escherichia coli WP2uvrA
|
Abstract:
Tall Oil, CAS No. 8002-26-4, was tested for mutagenic activity in Salmonella typhimurium strains TA 1535, TA 1537, TA 98 and TA 100 and Escherichia coli WP2uvrA at concentrations ranging from 17 to 5000 ?g per plate. The solvent used was dimethylsulphoxide. Two mutation assays (one direct plate and one pre-incubation) were conducted on agar plates in the presence and absence of an Aroclor 1254 induced rat liver preparation and the co-factors required for mixed-function oxidase activity (S9 mix). Concurrent positive controls demonstrated the sensitivity of the assay and the metabolising activity of the S9 mix. The results obtained in both assays were similar. No mutagenic activity was observed in any of the 5 bacterial strains, in either activation condition. Precipitation of the test item was observed in both mutation assays at 1667 and 5000 ?g per plate. Observable toxicity (a thinning of the background lawn of microcolonies) occurred in only one data set. This was in strain TA 1537 in the second mutation assay (pre-incubation) assay, in the absence of S9 mix only. Toxicity was observed at 1667 and 5000 ?g per plate. It was concluded that Tall Oil, CAS No. 8002-26-4, was not mutagenic to Salmonella typhimurium or Escherichia coli when tested in dimethylsulphoxide up to a predetermined maximum limit of 5000 ?g per plate.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Stevenson F M - Provided by: Walter Jones - ID: 100.IR.20338 - Date: 12/13/2001
Providing Organization: Pine Chemicals Association |
Document Title: Fatty acids, tall oil, CAS No. 61790-12-3Determination of Acute Toxicity (EL50) to Daphnia(48 h, Static)
|
Abstract:
This study was designed to determine the median effective loading (EL50) of Fatty acids, tall oil, CAS No. 61790-12-3, by exposing Daphnia under static conditions to solutions prepared as Water Accommodated Fractions (WAF) at different loading rates. This study was performed based on procedures in OECD (1984) Guideline 202 Part 1 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. Fatty acids, tall oil, a viscous brown liquid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organism to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive-limit tests, the test item was prepared and tested at a temperature appropriate to the test organisms as WAF, up to a maximum loading rate of 1000 mg.l 1. WAF were prepared in 2 litre capacity glass vessels with sidearm, with 2 litres of ASTM Daphnia medium. The vessels were placed on a magnetic stirrer and a stirrer bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 10 % of the water column. After a stirring period of ca 48 h the test solutions were allowed to settle for ca 1 h. The WAF was then removed via the sidearm taking care not to remove any undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test, a definitive-limit test was conducted at a maximum loading rate of 1000 mg.l 1, with an untreated control. No mortality or other effects were observed in the Daphnia during the study. The 48 h EL50 was > 1000 mg.l 1, the highest loading rate tested. The No Observed Effect Loading Rate (NOELr) was 1000 mg.l 1, under the conditions of the test. During the definitive-limit test, environmental conditions were within the following ranges, pH: 7.08 - 8.20, dissolved oxygen: 80.9 - 92.5 %, temperature: 21.0 - 22.2 ?C and conductivity 0.56 - 0.59 mS.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.20468 - Date: 6/14/2002
Providing Organization: Pine Chemicals Association |
Document Title: Fatty acids, tall oil, CAS No. 61790-12-3Determination of Acute Toxicity (EL50) to Daphnia(48 h, Sta
|
Abstract:
This study was designed to determine the median effective loading (EL50) of Fatty acids, tall oil, CAS No. 61790-12-3, by exposing Daphnia under static conditions to solutions prepared as Water Accommodated Fractions (WAF) at different loading rates. This study was performed based on procedures in OECD (1984) Guideline 202 Part 1 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. Fatty acids, tall oil, a viscous brown liquid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organism to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive-limit tests, the test item was prepared and tested at a temperature appropriate to the test organisms as WAF, up to a maximum loading rate of 1000 mg.l 1. WAF were prepared in 2 litre capacity glass vessels with sidearm, with 2 litres of ASTM Daphnia medium. The vessels were placed on a magnetic stirrer and a stirrer bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 10 % of the water column. After a stirring period of ca 48 h the test solutions were allowed to settle for ca 1 h. The WAF was then removed via the sidearm taking care not to remove any undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test, a definitive-limit test was conducted at a maximum loading rate of 1000 mg.l 1, with an untreated control. No mortality or other effects were observed in the Daphnia during the study. The 48 h EL50 was > 1000 mg.l 1, the highest loading rate tested. The No Observed Effect Loading Rate (NOELr) was 1000 mg.l 1, under the conditions of the test.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.20545 - Date: 6/18/2002
Providing Organization: Pine Chemicals Association |
Document Title: Fatty acids, tall oil, CAS No. 61790-12-3Determination of Acute Toxicity (LL50) to Fathead Minnows(96 h, Static)
|
Abstract:
This study was designed to determine the median lethal loading (LL50) of Fatty acids, tall oil, CAS No. 61790-12-3, by exposing fathead minnows (Pimephales promelas) under static conditions to solutions prepared as Water Accommodated Fractions (WAF) at different loading rates. This study was performed based on procedures in OECD (1992) Guideline 203 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. Fatty acids, tall oil, a viscous brown liquid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organisms to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive-limit tests, the test item was prepared and tested under conditions suitable to the test organisms as WAF, up to a maximum loading rate of 1000 mg.l-1. WAF were prepared in 20 litre capacity glass vessels, with 20 litres of Inveresk reconstituted freshwater medium. The vessels were placed on a magnetic stirrer and a stir bar added. The appropriate weight of Fatty acids, tall oil was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 10 % of the water column. After a stirring period of ca 48 h the test solutions were allowed to settle for ca 1 h. The WAF was then removed via a glass siphon taking care not to remove undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test a definitive-limit test was conducted at a maximum loading rate of 1000 mg.l 1, with an untreated control. No mortality or other effects were observed in the test fish during the study. The 96 h LL50 was > 1000 mg.l 1 the highest loading rate tested. The No Observed Effect Loading Rate (NOELr) was 1000 mg.l 1, under the conditions of the test. During the definitive-limit test, environmental conditions were within the following ranges, pH: 5.27 - 6.77, dissolved oxygen: 80.9 96.0 %, temperature: 22.5 24.9 ?C and conductivity: 0.17 - 0.24 mS. The light level in the test room was routinely measured as 640 lux (January 2002).
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.20621 - Date: 6/18/2002
Providing Organization: Pine Chemicals Association |
Document Title: Rosin, CAS No. 8050-09-7Determination of Acute Toxicity (EL50) to Daphnia(48 h, Static)
|
Abstract:
This study was designed to determine the median effective loading (EL50) of Rosin, CAS No. 8050-09-7, by exposing Daphnia under static conditions to solutions prepared as Water Accommodated Fractions (WAF) at different loading rates. This study was performed based on procedures in OECD (1984) Guideline 202 Part 1 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. Rosin, an amber crystalline solid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organism to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive tests, the test item was prepared and tested under conditions appropriate to the test organism as WAF, up to a maximum loading rate of 1000 mg.l-1. WAF were prepared in 2 litre capacity glass vessels with sidearm, with 2 litres of ASTM Daphnia medium. The vessels were placed on a magnetic stirrer and a stir bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 - 10 % of the water column. After a stirring period of ca 44 h for the range finding test and ca 48 h for the definitive test, the test solutions were allowed to settle for ca 1 h. The WAF was then removed via the sidearm taking care not to remove any undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test, a definitive test was conducted with WAF prepared at 1000, 750, 500, 250, 125, and 0 mg.l 1. After 24 h, 10 immobile Daphnia (50 %) were recorded at 1000 mg.l 1. A further 7 immobile Daphnia (85 %) were observed after the 48 h test period. The 48 h EL50 was 911 mg.l 1 and the No Observed Effect Loading Rate (NOELr) was 750 mg.l 1, under the conditions of the test. During the definitive test environmental conditions were within the following ranges, pH: 8.14 - 8.50, dissolved oxygen: 74.9 - 88.7 %, temperature: 19.3 23.2 ?C and conductivity 0.56 - 0.58 mS.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.20627 - Date: 6/7/2002
Providing Organization: Pine Chemicals Association |
Document Title: Rosin, CAS No. 8050-09-7Alga, Growth Inhibition Test(72 h, EL50)
|
Abstract:
This study was designed to determine the median effective loading (EL50) of Rosin, CAS No. 8050-09-7, by exposing alga (Selenastrum capriconutum) to solutions prepared as Water Accommodated Fractions (WAF) at different loading rates. This study was performed based on procedures in OECD (1984) Guideline 201 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. The test met all validity criteria. Rosin, an amber crystalline solid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organism to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive-limit tests, the test item was prepared and tested at a temperature appropriate to the test organism as WAF, up to a maximum loading rate of 1000 mg.l 1. WAF were prepared in 2 litre capacity glass vessels with sidearm, with 2 litres of ISO freshwater algal medium. The vessels were placed on a magnetic stirrer and a stir bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 - 10 % of the water column. After a stirring period of ca 48 h the test solutions were allowed to settle for ca 1 h. The WAF was then removed via the sidearm taking care not to remove any undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test a definitive-limit test was conducted at a maximum loading rate of 1000 mg.l 1, with an untreated control. As no effects or inhibition of growth was observed the 72 h EL50 for Area Under the Curve (AUC) and Average Specific Growth Rate (0-72 h) was > 1000 mg.l 1, the highest loading rate. Both the Average Specific Growth Rate and the AUC in the test treatments were greater than in the control group for 0 to 72 h. Hence the No Observed Effect Loading Rate (NOELr) for Average Specific Growth Rate and AUC was 1000 mg.l 1, under the conditions of the test. During the definitive-limit test, pH and temperature ranges were recorded as follows, pH: 7.6 9.31 (Table 4) and temperature: 24 - 26 ?C. The light intensities were 9450 lux (range finding test) and 8830 lux (definitive-limit test).
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.20681 - Date: 6/12/2002
Providing Organization: Pine Chemicals Association |
Document Title: Fatty acids, tall oil, CAS No. 61790-12-3 Alga, Growth Inhibition Test(72 h, EL50)
|
Abstract:
This study was designed to determine the median effective loading (EL50) of Fatty acids, tall oil, CAS No. 61790-12-3, by exposing alga (Selenastrum capriconutum) to solutions prepared as Water Accommodated Fractions (WAF) from different loading rates. This study was performed based on procedures in OECD (1984) Guideline 201 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. The test met all validity criteria. Fatty acids, tall oil, a viscous brown liquid is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organism to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive tests, the test item was prepared and tested at a temperature appropriate to the test organism as WAF, up to a maximum loading rate of 1000 mg.l 1. WAF were prepared in 2 litre capacity glass vessels with sidearm, with 2 litres of ISO freshwater algal medium. The vessels were placed on a magnetic stirrer and a stir bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 - 10 % of the water column. After a stirring period of ca 48 h the test solutions were allowed to settle for ca 1 h. The WAF was then removed via the sidearm taking care not to remove any undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test a definitive test was conducted at the initial loading rates of 1000, 750, 500, 250, 125 and 0 mg.l-1.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.20706 - Date: 6/14/2002
Providing Organization: Pine Chemicals Association |
Document Title: Fatty Acids, CAS No. 61790-12-3Chromosomal Aberration Assay with Chinese Hamster Ovary Cells in vitro(Complying with EC (Annex V) and OECD 473 Guidelines)
|
Abstract:
Fatty Acids, CAS No. 61790-12-3, was submitted for testing and evaluation of clastogenic potential. Chromosomal aberrations assays were performed with duplicate, Chinese hamster ovary (CHO) cell cultures. Dimethylsulphoxide was the vehicle and cyclophosphamide and methyl methanesulphonate were the positive controls used. The tests were conducted in the presence and absence of a post-mitochondrial supernatant fraction obtained from the livers of adult, male rats treated with Aroclor 1254 (S9) and a NADPH-generating system. Cultures, established approximately 20 hours before testing, were treated for 6 h in the presence and absence of S9 mix. Cultures were harvested at 24 h post treatment. Fatty Acids, CAS No. 61790-12-3, was toxic to Chinese hamster ovary cells in vitro in both the presence and absence of S9 mix. It was tested to the maximum permitted concentration of 5000 µg.ml-1 in the absence of S9 mix and up to 40 µg.ml-1 in the presence of S9 mix. Toxicity was noted at 20 40 µg.ml-1 in the presence of S9 mix and at 156 5000 µg.ml-1 in the absence of S9 mix. It was concluded that Fatty Acids, CAS No. 61790-12-3, was clastogenic when tested with Chinese hamster ovary cells in vitro in both the presence and absence of S9 mix. This response was observed only at concentrations deemed overtly toxic to the cells.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Murie E - Provided by: Walter Jones - ID: 100.IR.20712 - Date: 12/17/2001
Providing Organization: Pine Chemicals Association |
Document Title: Rosin, partially Hydrogenated methyl ester, CAS No. 8050-15-5Chromosomal Aberration Assay with Chinese Hamster Ovary Cells in vitro(Complying with EC (Annex V) and OECD 473 Guidelines)
|
Abstract:
Rosin, partially Hydrogenated methyl ester, CAS No. 8050-15-5, was submitted for testing and evaluation of clastogenic potential. Chromosomal aberrations assays were performed with duplicate, Chinese hamster ovary (CHO) cell cultures. This study was conducted incorporating two independent tests. Ethanol was the vehicle and cyclophosphamide and methyl methanesulphonate were the positive controls used in both tests. Both tests were conducted in the presence and absence of a post-mitochondrial supernatant fraction obtained from the livers of adult, male rats treated with Aroclor 1254 (S9) and a NADPH-generating system. Cultures, established approximately 20 hours before testing, were treated for 6 h in the presence or 6 and 22 hours in the absence of S9 mix. Cultures were harvested at 24 h (Test 1 and 2) or 48 h (Test 2) post treatment. Rosin, partially Hydrogenated methyl ester, CAS No. 8050-15-5, was toxic to Chinese hamster ovary cells in vitro in both the presence and absence of S9 mix. It was tested up to 40 µg.ml-1 in Test 1 and toxicity was noted at 20 40 µg.ml-1. In Test 2, Rosin, partially Hydrogenated methyl ester, CAS No. 8050-15-5, was tested up to 40 µg.ml-1 in the presence of S9 mix and up to 20 µg.ml-1 in the absence of S9 mix. Toxicity was noted in cultures treated with 20 40 µg.ml-1 (presence of S9 mix) and in cultures treated with 15 20 µg.ml-1 (absence of S9 mix). There was no evidence that Rosin, partially Hydrogenated methyl ester, CAS No. 8050-15-5, induced structural chromosomal aberrations in either the presence or absence of S9 mix. Rosin, partially Hydrogenated methyl ester, CAS No. 8050-15-5, did not induced an increase in polyploidy in the absence of S9 mix in cultures harvested 48 h post treatment. It was concluded that Rosin, partially Hydrogenated methyl ester, CAS No. 8050-15-5, was not clastogenic when tested with Chinese hamster ovary cells in vitro.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Murie E - Provided by: Walter Jones - ID: 100.IR.20718 - Date: 12/17/2002
Providing Organization: Pine Chemicals Association |
Document Title: Tall Oil, CAS No. 8002-26-4Chromosomal Aberration Assay with Chinese Hamster Ovary Cells in vitro(Complying with EC (Annex V) and OECD 473 Guidelines)
|
Abstract:
Tall Oil, CAS No. 8002-26-4, was submitted for testing and evaluation of clastogenic potential. Chromosomal aberrations assays were performed with duplicate, Chinese hamster ovary (CHO) cell cultures. This study was conducted incorporating two independent tests. Dimethylsulphoxide was the vehicle and cyclophosphamide and methyl methanesulphonate were the positive controls used in both tests. Both tests were conducted in the presence and absence of a post-mitochondrial supernatant fraction obtained from the livers of adult, male rats treated with Aroclor 1254 (S9) and a NADPH-generating system. Cultures, established approximately 20 hours before testing, were treated for 6 h in the presence or 6 and 22 hours in the absence of S9 mix. Cultures were harvested at 24 h (Test 1 and 2) or 48 h (Test 2) post treatment. Tall Oil, CAS No. 8002-26-4, was toxic to Chinese hamster ovary cells in vitro in both the presence and absence of S9 mix. It was tested to the maximum permitted concentration of 5000 µg.ml-1 in the absence of S9 mix and up to 80 µg.ml-1 in the presence of S9 mix (Test 1). Toxicity was noted at 60 and 80 µg.ml-1 in the presence of S9 mix and at 156 5000 µg.ml-1 in the absence of S9 mix. In Test 2, Tall Oil, CAS No. 8002-26-4, was tested up to 60 µg.ml-1 in the presence of S9 mix and up to 70 µg.ml-1 in the absence of S9 mix. Toxicity was noted in cultures treated with 30-60 µg.ml-1 (presence of S9 mix) and in cultures treated with 70 µg.ml-1 (absence of S9 mix). It was concluded that Tall Oil, CAS No. 8002-26-4, was weakly clastogenic when tested with Chinese hamster ovary cell in vitro in the presence of S9 mix. The response was observed only at a concentration level deemed overtly toxic to the cells.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Murie E - Provided by: Walter Jones - ID: 100.IR.20748 - Date: 12/17/2001
Providing Organization: Pine Chemicals Association |
Document Title: Rosin esters with Pentaerythritol, CAS No. 8050-26-8Determination of Acute Toxicity (LL50) to Fathead Minnows (96 h, Static)
|
Abstract:
This study was designed to determine the median lethal loading (LL50) of Rosin esters with Pentaerythritol, CAS No. 8050-26-8, by exposing fathead minnows (Pimephales promelas) under static conditions to solutions prepared as Water Accommodated Fractions (WAF) at different loading rates. This study was performed based on procedures in OECD (1992) Guideline 203 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. Rosin esters with Pentaerythritol, an amber crystalline solid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organisms to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive-limit tests, the test item was prepared and tested under conditions suitable to the test organisms as WAF, up to a maximum loading rate of 1000 mg.l-1. WAF were prepared in 20 litre capacity glass vessels, with 20 litres of Inveresk reconstituted freshwater medium. The vessels were placed on a magnetic stirrer and a stir bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 10 % of the water column. After a stirring period of ca 48 h the test solutions were allowed to settle for ca 1 h. The WAF was then removed via a glass siphon taking care not to remove undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test a definitive-limit test was conducted at a maximum loading rate of 1000 mg.l 1, with an untreated control. No mortality or other effects were observed in the test fish during the study. The 96 h LL50 was > 1000 mg.l 1, the highest loading rate tested. The No Observed Effect Loading Rate (NOELr) was 1000 mg.l 1, under the conditions of the test. During the definitive-limit test, environmental conditions were within the following ranges, pH: 6.67 7.08, dissolved oxygen: 88.6 91.7 %, temperature: 22.7 23.7 ?C and conductivity 0.19 - 0.21 mS. The light level in the test room was routinely measured as 640 lux (January 2002).
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.20784 - Date: 6/18/2002
Providing Organization: Pine Chemicals Association |
Document Title: Fatty acids, C18-Unsaturated dimers, CAS No. 61788-89-4Determination of Acute Toxicity (LL50) to Fathead Minnows(96 h, Static)
|
Abstract:
This study was designed to determine the median lethal loading (LL50) of Fatty acids, C18-Unsaturated dimers, CAS No. 61788-89-4, by exposing fathead minnows (Pimephales promelas) under static conditions to solutions prepared as Water Accommodated Fractions (WAF) at different loading rates. This study was performed based on procedures in OECD (1992) Guideline 203 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. Fatty acids, C18-Unsaturated dimers, an amber liquid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organisms to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive-limit tests, the test item was prepared and tested under a temperature suitable to the test organisms as WAF, up to a maximum loading rate of 1000 mg.l-1. WAF were prepared in 20 litre capacity glass vessels, with 20 litres of Inveresk reconstituted freshwater medium. The vessels were placed on a magnetic stirrer and a stir bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 10 % of the water column. After a stirring period of ca 48 h the test solutions were allowed to settle for ca 1 h. The WAF was then removed via a glass siphon taking care not to remove undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test a definitive-limit test was conducted at a maximum loading rate of 1000 mg.l 1, with an untreated control. No mortality or effects were observed in the test fish during the study. The 96 h LL50 was > 1000 mg.l 1, the highest loading rate tested. The No Observed Effect Loading Rate (NOELr) was 1000 mg.l 1, under the conditions of the test. During the definitive-limit test environmental conditions were within the following ranges, pH: 6.50 7.01, dissolved oxygen: 87.3 92.0 %, temperature: 22.5 23.9 ?C and conductivity: 0.19 - 0.20 mS. The light level in the test room was routinely measured as 640 lux (January 2002).
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.20785 - Date: 6/18/2002
Providing Organization: Pine Chemicals Association |
Document Title: Tall Oil, CAS No. 8002-26-4Determination of Acute Toxicity (LL50) to Fathead Minnows(96 h, Static)
|
Abstract:
This study was designed to determine the median lethal loading (LL50) of Tall oil, CAS No. 8002-26-4, by exposing fathead minnows (Pimephales promelas) under static conditions to solutions prepared as Water Accommodated Fractions (WAF) from different loading rates. This study was performed based on procedures in OECD (1992) Guideline 203 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. Tall oil, a dark brown liquid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organisms to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive-limit tests, the test item was prepared and tested under conditions suitable to the test organisms as WAF, up to a maximum loading rate of 1000 mg.l-1. WAF were prepared in 20 litre capacity glass vessels, with 20 litres of Inveresk reconstituted freshwater medium. The vessels were placed on a magnetic stirrer and a stir bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 10 % of the water column. After a stirring period of ca 48 h the test solutions were allowed to settle for ca 1 h. The WAF was then removed via a glass siphon taking care not to remove undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test a definitive-limit test was conducted at a maximum loading rate of 1000 mg.l 1, with an untreated control. No mortality or effects were observed in the test fish during the study. The 96 h LL50 was > 1000 mg.l 1, the highest loading rate tested. The No Observed Effect Loading Rate (NOELr) was 1000 mg.l 1, under the conditions of the test. During the definitive-limit test, environmental conditions were within the following ranges, pH: 6.48 7.00, dissolved oxygen: 86.2 91.7 %, temperature: 22.7 23.7 ?C and conductivity 0.19 - 0.20 mS. The light level in the test room was routinely measured as 640 lux (January 2002).
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.20787 - Date: 6/18/2002
Providing Organization: Pine Chemicals Association |
Document Title: Tall oil, CAS No. 8002-26-4 Alga, Growth Inhibition Test (72 h, EL50)
|
Abstract:
This study was designed to determine the median effective loading (EL50) of Tall oil, CAS No. 8002-26-4, by exposing alga (Selenastrum capriconutum) to solutions prepared as Water Accommodated Fractions (WAF) from different loading rates. This study was performed based on procedures in OECD (1984) Guideline 201 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. The test met all validity criteria. Tall oil, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organism to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive tests, the test item was prepared and tested at a temperature appropriate to the test organism as WAF, up to a maximum loading rate of 1000 mg.l 1. WAF were prepared in 2 litre capacity glass vessels with sidearm, with 2 litres of ISO freshwater algal medium. The vessels were placed on a magnetic stirrer and a stir bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 - 10 % of the water column. After a stirring period of ca 48 h the test solutions were allowed to settle for ca 1 h. The WAF was then removed via the sidearm taking care not to remove any undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test a definitive test was conducted with WAF prepared at 1000, 200, 40, 8, and 1.6mg.l-1, with an untreated control. As no effects or inhibition were observed the 72 h EL50 for Area Under the Curve (AUC) and Average Specific Growth Rate (0 72 h) was > 1000 mg.l-1, the highest loading rate tested. Both the Average Specific Growth Rate and the AUC in the test treatments were greater than in the control group for 0 to 72 h. Hence the No Observed Effect Loading Rate (NOELr) for Average Specific Growth Rate and AUC was 1000 mg.l 1, under the conditions of the test. During the definitive test, pH and temperature ranges were recorded as follows, pH: 6.47 - 8.38 (Table 4) and temperature: 22 - 24 ?C. The light intensities were 9200 lux (range finding test) and 5540 lux (definitive test).
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.20829 - Date: 6/7/2002
Providing Organization: Pine Chemicals Association |
Document Title: Rosin, esters with Pentaerythritol, CAS No. 8050-26-8Alga, Growth Inhibition Test (72 h, EL50)
|
Abstract:
This study was designed to determine the median effective loading (EL50) of Rosin, esters with Pentaerythritol, CAS No. 8050-26-8, by exposing alga (Selenastrum capriconutum) to solutions prepared as Water Accommodated Fractions (WAF) at different loading rates. This study was performed based on procedures in OECD (1984) Guideline 201 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. The test met all validity criteria. Rosin, esters with Pentaerythritol, an amber crystalline solid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organism to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive tests, the test item was prepared and tested at a temperature appropriate to the test organism as WAF, up to a maximum loading rate of 1000 mg.l 1. WAF were prepared in 2 litre capacity glass vessels with sidearm, with 2 litres of ISO freshwater algal medium. The vessels were placed on a magnetic stirrer and a stir bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 - 10 % of the water column. After a stirring period of ca 48 h the test solutions were allowed to settle for ca 1 h. The WAF was then removed via the sidearm taking care not to remove any undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test a definitive test was conducted with WAF prepared at 1000, 750, 500, 250, 125 and 0 mg.l 1. As no effects or inhibition of growth were observed, the 72 h EL50 for Area Under the Curve (AUC) and Average Specific Growth Rate (0 72 h) was > 1000 mg.l-1 the highest loading rate tested. The Average Specific Growth Rate and the AUC in the test treatments were not significantly different to the control group for 0 to 72 h. Hence the No Observed Effect Loading Rate (NOELr) for Average Specific Growth Rate and AUC was 1000 mg.l 1, under the conditions of the test.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.20834 - Date: 6/12/2002
Providing Organization: Pine Chemicals Association |
Document Title: Rosin, partially hydrogenated, methyl ester, CAS No. 8050-15-5Alga, Growth Inhibition Test(72 h, EL50)
|
Abstract:
This study was designed to determine the median effective loading (EL50) of Rosin, partially hydrogenated, methyl ester, CAS No. 8050-15-5 by exposing alga (Selenastrum capriconutum) to solutions prepared as Water Accommodated Fractions (WAF) at different loading rates. This study was performed based on procedures in OECD (1984) Guideline 201 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. The test met the validity criteria. Rosin, partially hydrogenated, methyl ester, amber liquid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organism to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive-limit tests, the test item was prepared and tested at a temperature appropriate to the test organism as WAF, up to a maximum loading rate of 1000 mg.l-1. WAF were prepared in 2 litre capacity glass vessels with sidearm, with 2 litres of ISO freshwater algal medium. The vessels were placed on a magnetic stirrer and a stir bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 - 10 % of the water column. After a stirring period of ca 48 h the test solutions were allowed to settle for ca 1 h. The WAF was then removed via the sidearm taking care not to remove any undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding, a definitive-limit test was conducted at a maximum loading rate of 1000 mg.l 1, with an untreated control. As no effects or inhibition of growth were observed, the 72 h EL50 for Area Under the Curve (AUC) and Average Specific Growth Rate (0 72 h) was > 1000 mg.l 1, the highest loading rate tested. Both the Average Specific Growth Rate and the AUC in the test treatments were greater than in the control group for 0 to 72 h. Hence the No Observed Effect Loading Rate (NOELr) for Average Specific Growth Rate and AUC was 1000 mg.l 1, under the conditions of the test.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.20840 - Date: 6/14/2002
Providing Organization: Pine Chemicals Association |
Document Title: Analytical Validation of Tall Oil in Diet (Rat and Mouse No. 3) Inveresk Report Number 20846
|
Abstract:
An analytical method for the determination of Tall Oil (CAS No. 8002-26-4) in Diet (Rat & Mouse No. 3) has been developed and validated at Inveresk (Analytical Method No. 1983). In the validated method, samples of formulated diet are extracted in Methanol and then diluted and spiked with an Internal Standard as necessary. Samples are analysed by Gas Chromatography. The chromatographic system has been shown to give linear response over the range ca 50 to ca 200 µg.ml-1 Tall Oil. The coefficient of correlation obtained was 0.9999. Analytical Method No. 1983 (Appendix 1) was found to be satisfactory over the range ca 800 p.p.m. to ca 20000 p.p.m. Tall Oil in Rat and Mouse No. 3 diet, in terms of linearity, assay specificity, limit of quantification, system precision, system suitability, assay accuracy and stability and assay precision. Samples prepared for chromatographic analysis were proven to be stable for at least 8 days when stored at ambient laboratory temperature in the dark. Formulation Method No. 1983 (Appendix 2), was found to be suitable for preparation of accurate and homogeneous rodent diets of Tall Oil at concentrations of ca 800 p.p.m. and ca 20000 p.p.m. Tall Oil. These formulations were found to be stable for at least 15 days for low level (800 p.p.m.) and at least 20 days for high level (20000 p.p.m.) when stored at ambient laboratory temperature in the dark.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Fernández R ~ Laing K - Provided by: Walter Jones - ID: 100.IR.20846 - Date: 2/14/2002
Providing Organization: Pine Chemicals Association |
Document Title: Fumarated Rosin, CAS No. 65997-04-8 Testing for Mutagenic Activity withSalmonella typhimurium
|
Abstract:
Fumarated Rosin, CAS No. 65997-04-8, was tested for mutagenic activity in Salmonella typhimurium strains TA 1535, TA 1537, TA 98 and TA 100 and Escherichia coli WP2uvrA at concentrations ranging from 17 to 5000 µg per plate. Two mutation assays (one direct plate and one pre-incubation) were conducted on agar plates in the presence and absence of an Aroclor 1254 induced rat liver preparation and the co-factors required for mixed-function oxidase activity (S9 mix). Concurrent positive controls demonstrated the sensitivity of the assay and the metabolising activity of the S9 mix. The results obtained in both assays were similar. No mutagenic activity was observed in any of the 5 bacterial strains, in either activation condition. There was no toxicity to the bacteria at any dose level. Precipitation of the test item was observed in both mutation assays at 5000 µg per plate in both the presence and the absence of S9 mix. At this dose level, precipitation was too dense to assess the condition of background lawns accurately for toxicity; however, the lawns were assumed to be normal. It was concluded that Fumarated Rosin, CAS No. 65997-04-8, was not mutagenic to Salmonella typhimurium or Escherichia coli when tested in dimethylsulphoxide up to a predetermined maximum limit of 5000 µg per plate.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Stevenson F M - Provided by: Walter Jones - ID: 100.IR.20912 - Date: 2/14/2002
Providing Organization: Pine Chemicals Association |
Document Title: Rosin, partially hydrogenated, methyl ester,CAS No. 8050-15-5 Acute Toxicity (LL50) to
|
Abstract:
This study was designed to determine the median lethal loading (LL50) of Rosin Fumarated, CAS No. 65997-04-8, by exposing fathead minnows (Pimephales promelas) under static conditions to solutions prepared as Water Accommodated Fractions (WAF) at different loading rates. This study was performed based on procedures in OECD (1992) Guideline 203 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. Rosin Fumarated, an amber solid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organisms to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive-limit tests, the test item was prepared and tested under conditions suitable to the test organisms as WAF, up to a maxmimum loading rate of 1000 mg.l-1. WAF were prepared in 20 litre capacity glass vessels, with 20 litres of Inveresk reconstituted freshwater medium. The vessels were placed on a magnetic stirrer and a stir bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 - 10 % of the water column. After a stirring period of ca 48 h the test solutions were allowed to settle for ca 1 h. The WAF was then removed via a glass siphon taking care not to remove undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test a definitive-limit test was conducted at a maximum loading rate of 1000 mg.l-1, with an untreated control. No mortality or other effects were observed in the test fish during the study. The 96 h LL50 was > 1000 mg.l-1, the highest loading rate tested. The No Observed Effect Loading Rate (NOELr) was 1000 mg.l-1, under the conditions of the test. During the definitive-limit test, environmental conditions were within the following ranges, pH: 7.48 7.84, dissolved oxygen: 73 95 %, temperature: 22.1 - 24.3 °C, and conductivity: 187.9 µS - 0.24 mS. The light level in the test room was routinely measured as 640 lux (January 2002).
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.20934 - Date: 6/18/2002
Providing Organization: Pine Chemicals Association |
Document Title: Rosin, partially hydrogenated methyl ester CAS No. 8050-15-5 Determination of Acute Toxicity (LL50)
|
Abstract:
This study was designed to determine the median lethal loading (LL50) of Rosin, partially hydrogenated, methyl ester, CAS No. 8050-15-5, by exposing fathead minnows (Pimephales promelas) under static conditions to solutions prepared as Water Accommodated Fractions (WAF) at different loading rates. This study was performed based on procedures in OECD (1992) Guideline 203 Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. Rosin, partially hydrogenated, methyl ester, an amber viscous liquid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organisms to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive-limit tests, the test item was prepared and tested under conditions suitable to the test organisms as WAF, up to a maximum loading rate of 1000 mg.l-1. WAF were prepared in 20 litre capacity glass vessels, with 20 litres of Inveresk reconstituted freshwater medium. The vessels were placed on a magnetic stirrer and a stir bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 - 10 % of the water column. After a stirring period of ca 48 h the test solutions were allowed to settle for ca 1 h. The WAF was then removed via a glass siphon taking care not to remove undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test a definitive-limit test was conducted at a maximum initial loading rate of 1000 mg.l-1, with an untreated control. No mortality or other effects were observed in the test fish during the study. The 96 h LL50 was > 1000 mg.l-1, the highest loading rate tested. The No Observed Effect Loading Rate (NOELr) was 1000 mg.l-1, under the conditions of the test. During the definitive-limit test, environmental conditions were within the following ranges, pH: 7.45 - 7.84, dissolved oxygen: 73 96 %, temperature: 22.1 - 24.1 °C and conductivity 189.7 µS - 0.24 mS. The light level in the test room was routinely measured as 640 lux (January 2002).
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.20948 - Date: 6/18/2002
Providing Organization: Pine Chemicals Association |
Document Title: Fatty acids, C18-Unsaturated dimers CAS No. 61788-89-4 Alga, Growth Inhibition Test (72 h, EL50)
|
Abstract:
This study was designed to determine the median effective loading (EL50) of Fatty acids, C18-Unsaturated dimers, CAS No. 61788-89-4, by exposing alga (Selenastrum capriconutum) to solutions prepared as Water Accommodated Fractions (WAF) at different loading rates. This study was performed based on procedures in OECD (1984) Guideline 201 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. The test met all validity criteria. Fatty acids, C18-Unsaturated dimers, an amber liquid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organism to insoluble fractions. The effects of both filtering and adjusting pH was investigated in the range finding test. For both the range finding and definitive-limit tests, the test item was prepared and tested at a temperature appropriate to the test organism as WAF, up to a maximum loading rate of 1000 mg.l 1. WAF were prepared in 2 litre capacity glass vessels with sidearm, with 2 litres of ISO freshwater algal medium. The vessels were placed on a magnetic stirrer and a stir bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 - 10 % of the water column. After a stirring period of ca 48 h the test solutions were allowed to settle for ca 1 h. The WAF was then removed via the sidearm taking care not to remove any undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test a definitive-limit test was conducted at a maximum loading rate of 1000 mg.l 1, with an untreated control. As no effects or inhibition of growth was observed the 72 h EL50 for Area Under the Curve (AUC) and Average Specific Growth Rate (0-72 h) was > 1000 mg.l 1, the highest loading rate tested. Both the Average Specific Growth Rate and the AUC in the test treatments were greater than in the control group for 0 to 72 h. Hence the No Observed Effect Loading Rate (NOELr) for Average Specific Growth Rate and AUC was 1000 mg.l 1, under the conditions of the test.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.20966 - Date: 6/7/2002
Providing Organization: Pine Chemicals Association |
Document Title: Determination of the Partition Coefficient of Fatty Acids and Fatty Acid Salts
|
Abstract:
Inveresk Research has carried out testing on Fatty Acid and Fatty Acid Salts to determine their partition coefficients. Testing was performed in accordance with OECD Guideline 117 (30 March 1989).
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Lightbody S M ~ Fisher K ~ Dinwoodie N B - Provided by: Walter Jones - ID: 100.IR.20976 - Date: 2/27/2003
Providing Organization: Pine Chemicals Association |
Document Title: Determination of the Partition Coefficient of Rosins, Rosin Salts, Rosin Adducts, Adduct Salts and Rosin Esters
|
Abstract:
Inveresk Research have carried out testing on Rosins, Rosin Salts, Rosin Adducts, Adduct Salts and Rosin Esters to determine their partition coefficients. Testing was performed in accordance with OECD Guideline 117 (30 March 1989). The HPLC Method (OECD 117) was selected for determination of partition coefficient over the Shake Flask Method (OECD 107) as some of the materials have surfactant properties. While these tests are designed for pure materials, all the materials tested are complex mixtures and hence result in multiple peaks. The table below outlines the results obtained for each material with a range of values given as appropriate. Test Material CAS Number Partition Coefficient Values Determined Range Main Peaks Rosin 8050-09-7 1.9-7.7 5.8, 6.4 Rosin, hydrogenated 65997-06-0 2.57.6 5.8, 7.0 Rosin, distillation overheads 68425-08-1 2.57.8 5.8, 7.0 Resin acids and Rosin Acids, potassium salt 61790-50-9 3.07.0 5.8, 6.5 Resin Acids and Rosin Acids, sodium salt 61790-51-0 3.56.6 5.8, 6.5 Rosin, maleated 8050-28-0 1.5-7.6 5.8, 6.4 Resin Acids and Rosin Acids, fumarated, sodium salt 68201-59-2 1.56.6 5.8, 6.4 Resin Acids and Rosin Acids, fumarated, potassium salt 68649-83-2 3.26.6 5.8 Resin Acids and Rosin Acids, maleated, potassium salt 85409-27-4 1.4-7.9 5.8, 6.4 Rosin, maleated/fumarated 68554-16-5 1.56.6 5.8, 6.4 Resin Acids and Rosin Acids, ester with diethyleneglycol 68153-38-8 4.05.8 4.8, 5.2 Resin Acids and Rosin Acids, methyl esters 68186-14-1 4.97.6 6.4, 7.1 Resin Acids and Rosin Acids, hydrogenated esters with glycerol 65997-13-9 4.75.8 4.7, 5.8 Resin Acids and Rosin Acids, hydrogenated esters with pentaerythritol 64365-17-9 4.67.3 4.6, 6.0, 6.1 Resin Acids and Rosin Acids, hydrogenated, methyl esters 8050-15-5 6.47.6 6.4, 7.6 Samples were initially chromatographed in unbuffered media (75:25 v/v Methanol/Milli-Q Water). However, a review of the chromatography obtained suggested that these materials might not be in the non-ionised form when chromatographed under these conditions. Therefore, testing was repeated using mobile phase adjusted to pH 2 with the exception of the esters which are unaffected by pH. In general, data from samples assayed at pH 2 showed better correlation within sample groups i.e. chromatography obtained from parent materials and salt forms were comparable. The results from this testing need to be viewed with caution as the method only covers materials with partition coefficient values in the range 0 to 6. Some components within the mixtures had estimated values outwith this range.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Lightbody S M ~ Fisher K ~ Dinwoodie N B - Provided by: Walter Jones - ID: 100.IR.20977 - Date: 2/27/2003
Providing Organization: Pine Chemicals Association |
Document Title: Determination of Partition Coefficient of Tall Oil and Tall Oil Related Products
|
Abstract:
Inveresk Research have carried out testing on Tall Oil and Tall Oil related products to determine their partition coefficients at the request of the Sponsor. Testing was performed in accordance with OECD Guideline 117 (30 March 1989). The HPLC Method (OECD 117) was selected for determination of partition coefficient over the Shake Flask Method (OECD 107) as some of the materials have surfactant properties. While these tests are designed for pure materials, all the materials tested are complex mixtures and hence result in multiple peaks. The table below outlines the results obtained for each material with a range of values given. Test Material CAS Number Partition Coefficient Values Estimated Determined Range Main Peak Tall Oil 8002-26-4 > 3.0 4.9 7.7 6.4 Tall Oil Pitch 8016-81-7 > 0.0 3.3 6.1 5.3 Tall Oil Pitch, sodium salt 68140-16-9 > 0.0 5.8 5.8 Tall Oil, disproportionated 68152-92-1 > 3.0 4.4 5.9 5.6 Tall Oil, sodium salt 65997-01-5 < - 2.0 4.9 7.6 7.0 Tall Oil, potassium salt 68647-71-2 < - 1.3 4.9 7.6 5.8 Samples were initially chromatographed in unbuffered media (75:25 v/v Methanol/Milli-Q Water). However, a review of the chromatograms obtained suggested that these materials may not be in the non-ionised form when chromatographed under these conditions. Therefore, testing was repeated using media adjusted to pH 2. In general, data from samples assayed at pH 2 showed better correlation within sample groups i.e. chromatography obtained from the parent materials and salt forms were comparable. The results from this testing need to be viewed with caution as the method only covers materials with partition coefficient values in the range 0 to 6. Some components within the mixtures had estimated values outside this range.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Lightbody S M ~ Fisher K ~ Dinwoodie N B - Provided by: Walter Jones - ID: 100.IR.20978 - Date: 4/4/2003
Providing Organization: Pine Chemicals Association |
Document Title: Determination of the Partition Coefficient of Dimer Acids
|
Abstract:
Inveresk Research have carried out testing on Dimer Acids to determine their partition coefficients. Testing was performed in accordance with OECD Guideline 117 (30 March 1989). The HPLC Method (OECD 117) was selected for determination of partition coefficient over the Shake Flask Method (OECD 107) as some of the materials have surfactant properties. While these tests are designed for pure materials, all the materials tested are complex mixtures and hence result in multiple peaks. The table below outlines the results obtained for each material with a range of values given and a weighted mean average as appropriate. Test Material CAS Number Partition Coefficient Values Determined Range Main Peaks Fatty Acids, C-18 unsaturated, dimers 61788-89-4 1-2.5 2.5 Fatty Acids, C-18 unsaturated, trimers 68937-90-6 2.2-8.9 2.2, 4.0, 6.8 Fatty Acids, C-18 unsaturated, dimers, hydrogenated 68783-41-5 0.7-6.2 2.5 Fatty Acids, C16-18 & C-18 unsaturated, dimerised 71808-39-4 2.47.5 4.3 Samples were initially chromatographed in unbuffered media (75:25 v/v Methanol/Milli-Q water). However, a review of the chromatograms obtained suggested that these materials may not be in the non-ionised form when chromatographed under these conditions. Testing was repeated using mobile phase adjusted to pH 2 and using acetonitrile as the organic component to increase solubility. The results from this testing need to be viewed with caution as the method only covers materials with partition coefficient values in the range 0 to 6. Some components within the mixtures may have values outwith this range
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Lightbody S M ~ Dinwoodie N B - Provided by: Walter Jones - ID: 100.IR.20979 - Date: 2/27/2003
Providing Organization: Pine Chemicals Association |
Document Title: Rosin Fumarated, CAS No. 65997-04-8 Alga, Growth Inhibition Test (72 h, EL50)
|
Abstract:
This study was designed to determine the median effective loading (EL50) of Rosin Fumarated, CAS No. 65997-04-8, by exposing alga (Selenastrum capriconutum) to solutions prepared as Water Accommodated Fractions (WAF) at different loading rates. This study was performed based on procedures in OECD (1984) Guideline 201 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. The test met all validity criteria. Rosin Fumarated, an amber solid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organism to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive-limit tests, the test item was prepared and tested at a temperature appropriate to the test organism as WAF, up to a maximum loading rate of 1000 mg.l 1. WAF were prepared in 2 litre capacity glass vessels with sidearm, with 2 litres of ISO freshwater algal medium. The vessels were placed on a magnetic stirrer and a stir bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 - 10 % of the water column. After a stirring period of ca 48 h the test solutions were allowed to settle for ca 1 h. The WAF was then removed via the sidearm taking care not to remove any undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test a definitive-limit test was conducted at a maximum loading rate of 1000 mg.l 1, with an untreated control. As no effects or inhibition of growth were observed the 72 h EL50 for Area Under the Curve (AUC) and Average Specific Growth Rate (0-72 h) was > 1000 mg.l 1, the highest loading rate tested. Both the Average Specific Growth Rate and the AUC in the test treatments were greater than in the control group for 0 to 72 h. Hence the No Observed Effect Loading Rate (NOELr) for Average Specific Growth Rate and AUC was 1000 mg.l 1, under the conditions of the test.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.21012 - Date: 6/14/2002
Providing Organization: Pine Chemicals Association |
Document Title: Tall oil, CAS No. 8002-26-4 Determination of Acute Toxicity (EL50) to Daphnia (48 h, Static)
|
Abstract:
This study was designed to determine the median effective loading (EL50) of Tall oil, CAS No. 8002-26-4 by exposing Daphnia under static conditions to solutions prepared as Water Accommodated Fractions (WAF) at different loading rates. This study was performed based on procedures in OECD (1984) Guideline 202 Part 1 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. Tall oil, a dark brown liquid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organism to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive-limit tests, the test item was prepared and tested at a temperature appropriate to the test organisms as WAF, up to a maximum loading rate of 1000 mg.l-1. WAF were prepared in 2 litre capacity glass vessels with sidearm, with 2 litres of ASTM Daphnia medium. The vessels were placed on a magnetic stirrer and a stir bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 - 10 % of the water column. After a stirring period of ca 48 h, the test solutions were allowed to settle for ca 1 h. The WAF was then removed via the sidearm taking care not to remove any undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test, a definitive-limit test was conducted at a maximum initial loading rate of 1000 mg.l 1, with an untreated control. No mortality or other effects were observed in the Daphnia during the study. The 48 h EL50 was > 1000 mg.l 1, the highest loading rate tested. The No Observed Effect Loading Rate (NOELr) was 1000 mg.l 1, under the conditions of the test. During the definitive-limit test, environmental conditions were within the following ranges, pH: 7.68 - 8.44, dissolved oxygen: 84 92 %, temperature: 20.4 22.8 ?C and conductivity: 0.58 0.67 mS.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.21015 - Date: 6/14/2002
Providing Organization: Pine Chemicals Association |
Document Title: Fatty acids, C18- Unsaturated dimers CAS No.61788-89-4 Determination of Acute Toxicity (EL50) to Dap
|
Abstract:
This study was designed to determine the median effective loading (EL50) of Fatty acids, C18-Unsaturated dimers, CAS No. 61788-89-4, by exposing Daphnia under static conditions to solutions prepared as Water Accommodated Fractions (WAF) at different loading rates. This study was performed based on procedures in OECD (1984) Guideline 202 Part 1 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. Fatty acids, C18-Unsaturated dimers, an amber liquid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organism to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive-limit tests, the test item was prepared and tested at a temperature appropriate to the test organism as WAF, up to a maximum loading rate of 1000 mg.l-1. WAF were prepared in 2 litre capacity glass vessels with sidearm, with 2 litres of ASTM Daphnia medium. The vessels were placed on a magnetic stirrer and a stir bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 - 10 % of the water column. After a stirring period of ca 48 h, the test solutions were allowed to settle for ca 1 h. The WAF was then removed via the sidearm taking care not to remove any undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test a definitive-limit test was conducted at a maximum initial loading rate of 1000 mg.l 1, with an untreated control. No mortality or other effects were observed in Daphnia during the study. The 48 h EL50 was > 1000 mg.l 1, the highest initial loading rate tested. The No Observed Effect Loading Rate (NOELr) was 1000 mg.l 1, under the conditions of the test. During the definitive-limit test environmental conditions were within the following ranges, pH: 7.84 8.29, dissolved oxygen: 76 82 %, temperature: 22.0 22.5 ?C and conductivity: 0.51 0.55 mS.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.21050 - Date: 6/14/2002
Providing Organization: Pine Chemicals Association |
Document Title: Rosin, esters with Pentaerythritol CAS No. 8050-26-8 Determination of Acute Toxicity (EL50) to Daphn
|
Abstract:
This study was designed to determine the median effective loading (EL50) of Rosin, esters with Pentaerythritol, CAS No. 8050-26-8, by exposing Daphnia under static conditions to solutions prepared as Water Accommodated Fractions (WAF) at different loading rates. This study was performed based on procedures in OECD (1984) Guideline 202 Part 1 for Testing of Chemicals and following procedures in OECD (2000) Guidance Document No. 23 on Testing Difficult Substances. Rosin, esters with Pentaerythritol, an amber solid, is a poorly soluble complex mixture. The method of preparation was selected to maximise the solubility of the test item under specific test exposure conditions, but reduce exposure of the test organisms to insoluble fractions. The effects of both filtering and adjusting pH were investigated in the range finding test. For both the range finding and definitive-limit tests, the test item was prepared and tested at a temperature appropriate to the test organisms as WAF, up to a maximum loading rate of 1000 mg.l-1. WAF were prepared in 2 litre capacity glass vessels with sidearm, with 2 litres of ASTM Daphnia medium. The vessels were placed on a magnetic stirrer and a stirrer bar added. The appropriate weight of the test item was added to the stirring medium and the vessels were sealed to avoid loss of volatile fractions. The stirring speed was adjusted to give a stirring vortex 5 10 % of the water column. After a stirring period of ca 48 h the test solutions were allowed to settle for ca 1 h. The WAF was then removed via the sidearm taking care not to remove any undissolved material at the top or bottom of the water column. The test organisms were exposed to the WAF. Following the results of the range finding test, a definitive-limit test was conducted at a maximum initial loading rate of 1000 mg.l 1, with an untreated control. No mortality or other effects were observed in the Daphnia during the study. The 48 h EL50 was > 1000 mg.l 1, the highest loading rate tested. The No Observed Effect Loading Rate (NOELr) was 1000 mg.l 1, under the conditions of the test. During the definitive-limit test environmental conditions were within the following ranges, pH: 8.12 8.23, dissolved oxygen: 75 82 %, temperature: 21.3 22.0 ?C and conductivity: 489 554 µS.
|
| |
Download Document:
Viewing this document is limited to PCA Membership Only
|
Author: Kelly C R ~ Clayton M A - Provided by: Walter Jones - ID: 100.IR.21051 - Date: 6/7/2002
Providing Organization: Pine Chemicals Association |
|
|
Today: Sunny with a high of 85
