Rosin, Partially Hydrogenated methyl ester, CAS No.8050-15-5,Testing for Mutagenic Activity with...
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Document Title:

Rosin, Partially Hydrogenated methyl ester, CAS No.8050-15-5,Testing for Mutagenic Activity with Salmonella typhimuriumTA 1535, TA 1537, TA 98 and TA 100 and Escherichia coli WP2uvrA

 

Abstract:

Rosin, Partially Hydrogenated methyl ester, CAS No. 8050-15-5, was tested for mutagenic activity in Salmonella typhimurium strains TA 1535, TA 1537, TA 98 and TA 100 and Escherichia coli WP2uvrA at concentrations ranging from 17 to 5000 ?g per plate. The solvent used was ethanol. Two mutation assays and a repeat test with TA 1537 and WP2uvrA were conducted on agar plates in the presence and absence of an Aroclor 1254 induced rat liver preparation and the co-factors required for mixed-function oxidase activity (S9 mix). Concurrent positive controls demonstrated the sensitivity of the assay and the metabolising activity of the S9 mix. No mutagenic activity was observed in any of the 5 bacterial strains, in either activation condition. Precipitation of the test item was observed in both mutation assays at 1667 and 5000 ?g per plate, in both activation conditions. There was no toxicity to the bacteria, although at 5000 ?g per plate, the precipitate was too dense to assess accurately the condition of the background lawns of microcolonies. However, the lawns were assumed to be normal. It was concluded that Rosin, Partially Hydrogenated methyl ester, CAS No. 8050-15-5, was not mutagenic to Salmonella typhimurium or Escherichia coli when tested in ethanol up to a predetermined maximum limit of 5000 ?g per plate.

 

Author: Stevenson F M - Provided by: Walter Jones - ID: 100.IR.20337 - Date: 12/13/2001

Providing Organization: Pine Chemicals Association

 

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