|Tall Oil [distilled tall oil], CAS No. 8002-26-4Testing for Mutagenic Activity with Salmonella ...|
Tall Oil [distilled tall oil], CAS No. 8002-26-4Testing for Mutagenic Activity with Salmonella typhimuriumTA 1535, TA 1537, TA 98 and TA 100 and Escherichia coli WP2uvrA
Tall Oil [distilled tall oil], CAS No. 8002-26-4, was tested for mutagenic activity in Salmonella typhimurium strains TA 1535, TA 1537, TA 98 and TA 100 and Escherichia coli WP2uvrA at concentrations ranging from 17 to 5000 ?g per plate. The solvent used was dimethylsulphoxide. Two mutation assays (one direct plate and one pre-incubation) were conducted on agar plates in the presence and absence of an Aroclor 1254 induced rat liver preparation and the co-factors required for mixed-function oxidase activity (S9 mix). Concurrent positive controls demonstrated the sensitivity of the assay and the metabolising activity of the S9 mix. The results obtained in both assays were similar. No mutagenic activity was observed in any of the 5 bacterial strains, in either activation condition. Precipitation of the test item was observed in both mutation assays at 1667 and 5000 ?g per plate. Observable toxicity (a thinning of the background lawn of microcolonies) occurred in only one data set. This was in strain TA 1537 in the second mutation assay (pre-incubation) assay, in the absence of S9 mix only. Toxicity was observed at 1667 and 5000 ?g per plate. It was concluded that Tall Oil, CAS No. 8002-26-4, was not mutagenic to Salmonella typhimurium or Escherichia coli when tested in dimethylsulphoxide up to a predetermined maximum limit of 5000 ?g per plate.
Author: Stevenson F M - Provided by: Walter Jones - ID: 100.IR.20338 - Date: 12/13/2001
Providing Organization: Pine Chemicals Association
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